Long-term Culture, Blastocyst Culture

Sumiyoshi Ginza

This is the method of transfer and cryopreservation of the developed embryo and blastocyst after OPU and culturing to Day 4, 7.
We will be able to remove the embryos that stop splitting in the early term and strictly sort the good embryos.
Early embryos and post-compaction (day 3-4) embryos and blastocysts require different nutrients, so during long-term culture, change the type of culture medium on day 3.
Recently, the culture medium has been improved, and there is also a medium that can be cultured from early embryos to blastocysts.

Flow of Long-term Culture・Blastocyst Culture

Day 0	Oocyte Pick-Up Conventional IVF or ICSI Fertilization
Day 1	Pronuclear Period	Pyruvic acid is the source of energy of the early embryo Culture Solution including much pyruvic acid Organization close to the fallopian tubal fluid of people
Day 2
	Period of Cell Division
Day 3
Day 4	Compaction	In the case of people it will become a compaction after 16cells and the energy source will change to glucose Culture Solution including much glucose Organization close to the uterus
Day 5	Blastocyst Early Blastocyst
	Expanded Blastocyst
	Hatched Blastocyst

	Implantation & Pregnancy